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Les mecs sont arrivés à dé-cuire un œuf

29/01/2015 | Etudes Compléments alimentaires


Je ne vois pas trop les applications mais visiblement, on risque de se retrouver avec des prot de lait dé-cuites

Shear-Stress-Mediated Refolding of Proteins from Aggregates and Inclusion Bodies
Dr. Tom Z. Yuan             ChemBioChem Early View (Online Version of Record published before inclusion in an issue) 23 JAN 2015

Recombinant protein overexpression of large proteins in bacteria often results in insoluble and misfolded proteins directed to inclusion bodies. We report the application of shear stress in micrometer-wide, thin fluid films to refold boiled hen egg white lysozyme, recombinant hen egg white lysozyme, and recombinant caveolin-1. Furthermore, the approach allowed refolding of a much larger protein, cAMP-dependent protein kinase A (PKA). The reported methods require only minutes, which is more than 100 times faster than conventional overnight dialysis. This rapid refolding technique could significantly shorten times, lower costs, and reduce waste streams associated with protein expression for a wide range of industrial and research applications.

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