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23/08/2018 | Etudes Compléments alimentaires

 

Excess of nutrient-induced morpho-functional adaptation and inflammation degree in a Caco2/HT-29 in vitro intestinal co-culture
M. Bottani,        j.nut.2018.07.018

Highlights
•A nutrient excess increases in vitro mucus production and reduces tight junctions.
•A nutrient excess increases the specific activity of intestinal enzymes.
•A nutrient excess increases the intestinal permeability.
•A higher ROS, NO and PYY hormone production is associated to a nutrient excess.
•A low grade inflammation status is induced by a nutrient excess.

Abstract
The intestinal cell function can be modulated by the type and quantity of nutrients. The aim of this study was to evaluate the effects due to an excess of nutrients on intestinal morpho-functional features and a possible associated inflammation in a 70/30 Caco2/HT-29 intestinal in vitro co-culture. An excess of nutrients (EX) has been obtained by increasing progressively the medium change frequency respect to standard cell growth conditions (ST) from confluence (T0) to 15 days after confluence (T15). In comparison with ST group, the EX group revealed a maintenance of the number of microvilli, an increase of follicle like-structures and mucus production, a decrease in the number of tight junction. The specific activity of markers of intestinal differentiation, Alkaline Phosphatase and Aminopeptidase N, and of the enterocyte differentiation specific marker, Dipeptidyl Peptidase-IV, progressively raised. The Transepithelial Electrical Resistance, indicative of the co-culture barrier properties, decreased, while Lucifer Yellow Papp evaluation, an index of the paracellular permeability to large molecules, showed an increase. ROS and NO production, indicative of an oxidative status, together with IL-6, IL-8, indicative of a low inflammation grade, and PYY secretion were higher in EX than in ST condition. The differences between ST and EX were particularly evident at T15.

These data support the suitability of our in vitro gut model for obesity studies at the molecular level and the necessity to standardize the medium frequency change in intestinal culture.

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