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Protéines: plus chère ne veut pas dire plus anabolique

04/05/2014 | Etudes Compléments alimentaires et Etudes Perte de poids et Etudes Anti-âge

 

Differential effects of whey protein concentrate and hydrolyzed whey/egg protein blends on post-prandial markers of insulin signaling and skeletal muscle anabolism in rats
C. Brooks Mobley       April 2014 The FASEB Journal vol. 28 no. 1 Supplement LB439

We examined how gavage-feeding the following dietary proteins (10 human eq. g dissolved in 1 ml of water) acutely affected skeletal muscle insulin signaling and anabolism markers 90- and 180-min post-feeding in male Wistar rats (~250 g) fasted overnight.

Supplementation included:
a) 80% whey protein concentrate (WPC, n = 15);
b) 70% hydrolyzed whey + 30% hydrolyzed egg albumin (70W/30E, n = 15);
c) 50W/50E (n = 15);
d) 30W/70E (n = 15); and
e) 1 ml of water with no protein (fasted, n = 14).

Immunoblotting results from mixed gastrocnemius muscles revealed:
1) rats fed WPC, 70W/30E and 50W/50E typically presented an in phospho-Akt (Thr308) compared to 30W/70E and fasting rats (p < 0.05);
2) markers of mTOR signaling (p-mTOR Ser2481, p-p70s6k Thr389, p-rpS6 Ser235/236) were typically greater in rats fed 70W/30E and 50W/50E compared to WPC, 30W/70E and fasting rats (p < 0.05); and
3) 180-min post-feeding muscle protein synthesis (MPS) analysis demonstrated that, compared to fasted rats, WPC feeding increased MPS by 94% (p < 0.05),
70W/30E feeding increased MPS by 74% (p = 0.065) and
50W/50E and 30W/70E feedings non-significantly increased MPS by 25% and 34%, respectively (p > 0.05).

In summary, 70W/30E feeding elicits similar acute post-feeding responses to that of WPC. However, replacing hydrolyzed whey with a higher percentage of hydrolyzed egg albumin attenuated these responses.

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